Photosynthesis Non Cyclic Electron Flow and Calvin Cycle Amusical Lecture Glenn Wolkenfeld for joining us for Episode Two, Wher more photosynthesic waits for you. We last saw how cells create, O¬¬2, ATP, and NADPH. Now we’ll start with a twist on light reactions, Called flow, we’ll see how it happens. we will investigate how the Calvin Cycle makes carbohydrate. (Part I, Cyclic Electron Non – cyclic flow is also called the “Z – scheme” Z on its site and see what I mean. Electrons rise energy then fall and rise again. Making ATP and my friends. A variation on this cyclic flow. Which makes ATP, no NADPH or Cyclic flow results the Calvin Cycle’s needs. It less NADPH than ATP. NADPH buildup has the repercussion, inhibiting NADP+ reduction. ‘cause clogs up reductase. NADP+ from moving into place. electrons don’t go to NADP+. clogged, stuck like a pimple’s pus. Where can go from P700? Nature evolved them a place flow. A shunt pathway moves them the ETC. I’m talking ‘bout the one in PS II tou These energized alectrons flow release energy. as you know is used to synthesize ATP. ( Part Calvin Cycle) Now it’s time to learn the cycle named for Which results the fixation of carbon. Consider that each carbon in bones, blood and hair, once in a CO2 in the air. The Calvin has three parts. of carbon is how it starts. The molecules produced then reduced. ‘cause reduction is the key thing that happens in 2. Part 2 is the as you will see. It produces G3P A molecule with lots of energy. And a starting point for all molecules that cells need. Part 3 sets up conditions for the cycle’s By recreating at the cycle’s heart. RuBP (Ribulose Bisphosphate), a five carbon molecule that seems to ‘Cause RuBP’s the cycle’s start. Then reappears at the very part. The role of RuBP at the start and Is why this is a cycle like the one Krebs. The cycle’s inputs are know you: NADPH and ATP and CO2 Fixation of carbon is catalyzed RuBP Carboxylase Enzyme enzyme’s nickname you should know. Sounds like a cookie it’s RUBISCO. An enzyme so old it was already O2 accumulated in the air. The CO2 enters through leaf stoma, through the leaf and arriving at the stroma. There RuBisCO grabs it in its active site, and squeezes with RuBP tight. RuBP has carbons five, it has two phosphates, one on side. When CO2 combines RuBP it makes a six carbon compound you won’t see. It instantly dissociates into two 3 – Each three carbons and one phosphate. Each phosphorylated to bisphosohoglycerate. Phosohorylated means that each receives aphosphate group from phospharylation, is followed by reduction. And you know an enzyme with the function. Of taking electrons and H from NADPH and them to to bisphosphoglycerate. Take off a phosphate for – Phosphate. A point for compounds like carbohydrates. (Part III: Cycle, conclusion) Glyceraldehyde- 3 – phosphat, as G3P, a product cells harvest, and I hope you see, how light NADPH and ATP, are needed to fix CO2 make organic energy. And to the cycle we must now regenerate. The starting of Calvin Cycle ribulose bisphosphate. The involved in taking G3Ps, And recombining them into Are too much freshman biology. You can get the details when you get Ph. D. But know for now that to get 3 from 5 G3Ps cost 3 ATPs! (Part IV: Conclusion) Now this journey whit its emphasis, the reaction of photosynthesis. For over billion years it’s been the food chains basis. In from that era you can see the traces, Of tiny with prokaryotic form. Stromatolites lived in waters warm. Through they transformed our planet, So aerobic can live upon it. so evolved sentient beings who can analyze, photoautotrophs can photosynthesize. You could say that photosynthesis gave birth to codiyions cons ciousness on Planet Earth!