Photosynthesis Cyclic Electron Flow and The Calvin Cycle Amusical Lecture by Glenn Thanks for joining us for Two, Wher more ‘bout photosynthesic waits you. We last how plant cells create, O¬¬2, ATP, and NADPH. Now we’ll with a twist on the light reactions, cyclic flow, we’ll see how it happens. Then we will investigate how the Calvin Cycle carbohydrate. I, Cyclic Electron Flow) Non – flow is also called the “Z – scheme” Put Z on its site and see what I mean. Electrons rise in energy then and rise again. Making ATP and NADPH friends. A variation on this is flow. Which makes no NADPH or O. flow results from the Calvin Cycle’s needs. It uses NADPH than ATP. NADPH buildup the repercussion, of inhibiting NADP+ reduction. ‘cause NADPH clogs up Keepping NADP+ from moving into So electrons don’t go NADP+. They’re stuck like a pimple’s pus. Where can they go from Nature evolved a place to flow. A shunt pathway them to the ETC. I’m talking ‘bout the in PS II tou see. These alectrons flow and release energy. Which as you know is used synthesize ATP. ( Part Calvin Cycle) Now it’s to learn the cycle named for Calvin. Which in the fixation of carbon. Consider that each carbon in your bones, blood and Was in a CO2 in the air. The Calvin has three parts. Fixation of carbon is it starts. The molecules produced rae reduced. reduction is the key thing that happens in part 2. Part 2 is the payoff as will see. It produces G3P A reduced molecule lots of energy. And a starting point for all the molecules cells need. Part sets up conditions for the cycle’s start. By recreating what’s at the heart. (Ribulose Bisphosphate), a five carbon molecule that seems to apparate. ‘Cause RuBP’s the cycle’s start. Then reappears at the very last role of RuBP at the start and end. Is this is a cycle like the one named Krebs. The cycle’s inputs are know to you: NADPH and and CO2 Fixation carbon is catalyzed by RuBP Carboxylase Enzyme This enzyme’s nickname you know. Sounds like cookie maker: it’s RUBISCO. An enzyme so old it was here, Before O2 in the air. The CO2 enters through leaf stoma, diffusing the leaf and arriving at the stroma. There RuBisCO grabs it in its active site, and squeezes it with RuBP RuBP has carbons five, it has two one on each side. When CO2 combines with RuBP it makes a carbon compound you won’t see. It instantly dissociates two 3 – phosphoglycerates, Each with three carbons and phosphate. Each gets phosphorylated bisphosohoglycerate. Phosohorylated that each receives aphosphate group from ATP. This phospharylation, is followed by And you know an enzyme with the function. Of taking electrons and H from NADPH and adding them to to off a phosphate for Glycealdehyde – Phosphate. A starting for compounds like carbohydrates. (Part III: Cycle, conclusion) Glyceraldehyde- 3 phosphat, known as G3P, a product cells harvest, and I hope you see, how light reactions and ATP, are needed to fix and make organic energy. And to complete the cycle must now regenerate. The starting point Calvin Cycle ribulose bisphosphate. The intermediates involved taking G3Ps, And recombining them RuBPs. Are too for freshman biology. You can get the details when you get yors Ph. But know for now that to get 3 RuBPs from 5 cost 3 ATPs! (Part IV: Now this journey ends its emphasis, On the reaction of For over four billion years it’s been the chains basis. In fossils from that era you can see traces, tiny cells with prokaryotic form. Stromatolites that lived in waters Through photosynthesis they transformed planet, So aerobic life can upon it. And so evolved sentient beings who analyze, How photoautotrophs can You could say that photosynthesis gave to codiyions for cons ciousness on Planet Earth!