Photosynthesis : part - The Light Reactions Lecture by Glenn Wolkenfeld A music Lecture by Glenn Boys and girls me what you think of this, Time for lesson ‘bout photosynthesis Cells use light combine water carbon dioxide. see electrons going for a wild ride. Making sugar, sweet O¬2. But should photosynthesis matter to you? Food your table. rich atmosphere. Without photosynthesis simple wouldn’d be here. ( I, The Big Picture) Photosynthesis a redox reaction. by light using enzymatic action. Water’s oxidized, CO2’s duced The sugar that results drink in orange juice. The overall reaction two phases. First is light reactions which has as its basis. powered production of ATP. O2 is the – product and you will see. Production of carrier NADPH Which provides reducing power needed in second phase ‘Cause NADPH, during phase Provides needed for reducing CO2 NADPH ia made by a reduction NADP+, whose function Is to absorb and pick up H, Gaining energy and NADPH. Phase is the Calvin Cycle makes carbohydrate, Like sugar and starches and fibers on your plate. how plants make food animals eat. Photosynthesis, it’s so (Part Chloroplasts, Thylakoids, Light, and Chlorophyll) In a lesf there’s mesophyllic And cells with chloroplasts key the issue. Chloroplasts are almost like cells every way, With their ribosomes and their own DNA. They even reproduce splitting into half. A clue for this independent past. This theory has a name Developed in the by Lynn Margulis. Inside the chloroplast is the stroma. It’s true in It’s true Roma. Stroma syrrounds thylakoids. one has a hollow space inside. thylakoid’s membrane is loaded with protein. ‘Cause it’s the reactions’ scene. It’s all about light for powering electrons, All about grabbing the power in The needed are are those from light, Which the energy to make the world bright. The frequency that we can see, violet light with wavelength 380. nanometers, sounds small, But next to rays it’s rather tall. Red shines at 750. Isn’t the visible nifty? Photons are packets of light Both a wave and a particle, you get adsorbed by pigments. It’s real, it’s true, ain’t no figment! Most important green chlorophylls. Orange carotenoids, yellow xanthophylls. Together absorb light, mostly red and blue. The green is not absorbed reflects back to you. In chlorophyll see the ring. the magnesium, which makes you want to sing, metal with valence two. You’ll see electrons rocketing through. Notice the tail, hydrocarbon chain. Which anchors into the thylakoid membrane. molecule with style, so pretty, In the thylakoid makes electricity! ( III) Englemann showed the action spectrum. a prism to break the sun’s Light into frequencies. Shone it on filamentous algae. The O2 algae make stimulate bacteria. Which will grow in any oxygen – rich Bacteria loved it over blue and But hardly grew over green, they might as be dead! The line of growth a refection, of photosynthesis’ action spectrum. Note won’t match, exactly, absorption spectra of chlorophyll a or b. the cartenoids and xanthophylls we’ve met, Absorb light frequencies chlorophylls can’t get. And the thylakoid they all cooperate, In light to synthesize carbohydrate. Consider chlorophyll isolation, Shine some light upon it it photoexcitation) The valence electrons magnessium Jump to an state, it’s so fun! But once up at that they can only fall. to where they started, like a bouncing ball. These falling release energy, Fluorescing as red light, as can see. in thylakoids, chlorophyll’s not alone, Instesd a photosystem is home. The photosystem’s parts can take And use their energy move electrons. The system’s antenna complex does first capture, Changing anergy into electron rapture. The energized electrons bounce like ball, At the reaction center, they but do not fall. A primary electron acceotor grabs them on top, And yanks them with a force reaction center cannot To this oxidized center we’ll return, But the details of electron flow it now is to learn. ( Part ATP Synthesis in Non – Cyclic Electron Flow. Non - cycle flow is the pathway. Much confustion relates the way II precedes photosystem I. It’s a fct to just get it done! PS II’s antenna a photon, Generates a flow electrons. All the world, even n Haiti, They flow reaction center P680. There electrons do not acceptor passes them over. the electron transport chain, In the membrane. This chain’s like a brigade. Each molecule has a trade. They take energized And use their energy for pumping From stroma to the space. Packing into that place. This makes a gradient across the thylkoid The protons want “out” or go insane. the protons can’t permeate. The membrane won’t allow There’s only one port through which they It’s ATP Synthase channel. The “-ase” tells this is an enzyme. Which makes all the time. It binding sites for ADP and Pi, And channels for diffusing protons whose energy. Changes the binding conformation. Fusing ADP Pi which for your information. Is thylakoids make ATP. key form of energy. From this might feel familiar. ATP is made this way mitochondria. is the name production is the game. ( V: NADPH Synthesis in Non – Cyclic Elecron Flow) Back our electons moved by the sun. from Photosystem II to System I. They to PS I lke a used up battery. transport chain used up their energy. But when light hits I’s antenna boune to reaction center. This P700, That wavelength makes electrons T’s electron acceptor grabs them away, Leaving P700 in an oxidized And P700 with its blues accepts the alectrons from PS II. to electron from PS I. It now gose for ride, having its own fun. But powering proton ain’t its fate. travels to NADP+ reductase. An enzyme plant count upon. To some H and electrons. To NADP+ gets reduced. how NADPH get produced. (Part VI: Oxygen Production in – Cycli Electron Flow) The by product of these light reactions Is here’s how it happens. Back PS II, came to arise, That P680 oxidized To replace lost electrons liberates. Electrons water which dissociates. Into one oxygen and protons single O will meet anoher forming O2. These are formed in the thylakoid space. Increasing concentration in that place. So we see a side effect of water’s enhancement of ATP creation.