Photosynthesis : part A - The Light Amusical by Glenn Wolkenfeld A music Lecture by Wolkenfeld Boys and girls tell me what you think of Time for lesson ‘bout photosynthesis use light to combine water carbon dioxide. Gonna see electrons going a wild ride. sugar, making sweet O¬2. why should photosynthesis matter to you? Food on your O2 rich Without photosynthesis simple wouldn’d be here. ( Part I, Big Picture) Photosynthesis a redox reaction. by light using enzymatic action. oxidized, CO2’s re duced The sugar that results you drink in juice. The overall reaction has phases. First the light reactions which has as its basis. Light production of ATP. O2 is the – product and you will see. Production of electron carrier Which provides reducing power in the second phase NADPH, during phase 2 Provides needed for reducing CO2 NADPH ia made by a reduction of whose function Is to absorb electrons pick up H, Gaining energy and NADPH. Phase 2 is the Calvin Cycle carbohydrate, Like sugar and starches and fibers on your plate. It’s how make food animals eat. it’s so sweet! (Part II: Thylakoids, Light, and Chlorophyll) In a lesf there’s tissue And with chloroplasts key to the issue. Chloroplasts are almost like cells in way, With their own and their own DNA. They even reproduce themselves, into half. A clue for this organelle’s independent This has a name endosymbiosis, Developed in 60s by Lynn Margulis. Inside the is the fluid stroma. It’s true in It’s in Roma. Stroma syrrounds little Each one has a hollow space The thylakoid’s membrane is loaded with ‘Cause it’s the light scene. It’s all about using light for electrons, All about the power in photons. The needed are are those from light, Which has the energy to the world bright. The highest frequency that can see, Is violet light wavelength 380. 380 nanometers, small, But next to gamma rays rather tall. Red light shines at Isn’t visible spectrum nifty? are packets of light energy, Both a wave and particle, you see. Phootins get adsorbed pigments. It’s real, it’s true, ain’t no figment! Most important are green Orange carotenoids, yellow xanthophylls. Together they absorb light, mostly red and green is not absorbed it reflects back to you. chlorophyll see the porphyrin ring. See the magnesium, which makes you want to metal with valence two. see those electrons rocketing through. Notice the tail, a chain. Which chlorophyll into the thylakoid membrane. A molecule with style, pretty, the thylakoid it makes electricity! ( III) showed us the action spectrum. a prism to break the sun’s into its frequencies. Shone it on spirogyra, algae. O2 algae make will stimulate bacteria. Which will grow in any oxygen rich area. loved it over blue and red, But hardly grew over green, might as well be dead! The line of growth is a refection, of action spectrum. Note this match, exactly, The absorption spectra of chlorophyll or b. ‘Cause the cartenoids xanthophylls we’ve met, Absorb light frequencies the chlorophylls get. And in thylakoid they all cooperate, using light to synthesize carbohydrate. Consider in isolation, Shine light upon it (call it photoexcitation) The electrons in magnessium Jump to an state, it’s so fun! But once up at that level, can only fall. Back to where they started, like bouncing ball. These falling alectrons, energy, Fluorescing red light, as you can see. But thylakoids, chlorophyll’s not alone, Instesd photosystem is chlorophyll’s home. The parts can take photons, And use energy to move electrons. The system’s antenna complex does the first Changing photon into electron rapture. The electrons bounce like a ball, At the reaction center, they jump but not fall. A electron acceotor grabs them from on top, And yanks them with a force center cannot stop. To this reaction center we’ll return, But the details of electron flow it now is to learn. ( Part IV) ATP Synthesis in – Cyclic Electron Flow. Non - cycle is the main pathway. Much confustion relates to way Hootoystm II precedes photosystem It’s fct to memorize, just get it done! PS II’s antenna captures a Generates a flow of All around the even n Haiti, They flow to reaction center There electrons do hover, acceptor passes them over. To the transport chain, In the membrane. This chain’s like a brigade. Each molecule has a similar They take energized use their energy for pumping protons. From stroma to thylakoid space. Packing protons that place. This makes a gradient the thylkoid membrane The protons want “out” or they’ll insane. But the protons permeate. The membrane won’t allow There’s only one port through they travel. It’s the ATP channel. The “-ase” tells you is an enzyme. Which makes all the time. It has binding sites ADP and Pi, And channels for diffusing protons whose knetic the binding sites’ conformation. Fusing ADP with Pi for your information. Is how make ATP. Life’s key of energy. From this might feel familiar. ATP is made way in mitochondria. Chemiosomosis is name production is the game. ( Part V: NADPH Synthesis in Non Cyclic Elecron Flow) Back to our electons moved by the Flowing from II to System I. They get to PS lke a used up battery. The chain used up their energy. But when hits PS I’s antenna Electrons boune reaction center. one’s P700, That makes electrons go! T’s electron acceptor grabs them away, Leaving P700 in oxidized way. And P700 with its oxidized blues the alectrons from PS II. to electron from PS I. It gose for a ride, having its own fun. But proton pumps ain’t its fate. It to NADP+ reductase. An enzyme plant cells count To pass some H and To NADP+ which gets It’s how get produced. (Part VI: Oxygen Production in – Cycli Electron Flow) The by – product of these reactions Is oxygen how it happens. Back PS it came to arise, That P680 got To replace lost electrons P680 from water which dissociates. Into one oxygen protons two, The single O will meet anoher forming These are formed in the thylakoid space. Increasing proton in that place. So we see a side effect water’s oxidation. Is enhancement of ATP