Photosynthesis part A - The Light Reactions Amusical by Glenn Wolkenfeld A Lecture by Glenn Wolkenfeld Boys and girls tell what you think of this, for a lesson ‘bout photosynthesis Cells use light to combine carbon dioxide. Gonna see electrons for a wild ride. Making sugar, making O¬2. But why should photosynthesis matter to Food your table. O2 rich Without photosynthesis you simple wouldn’d here. ( I, The Big Picture) is a redox reaction. Powered by light enzymatic action. oxidized, CO2’s re duced The sugar that results you drink in juice. overall reaction has two phases. First the light reactions which has as its basis. Light powered of ATP. O2 is the by – and you will see. of electron carrier NADPH Which provides reducing power needed in second phase NADPH, during phase 2 Provides what’s needed reducing CO2 NADPH made by a reduction of NADP+, whose function Is to absorb electrons and up H, energy and making NADPH. Phase 2 the Calvin Cycle makes carbohydrate, Like sugar and starches and fibers on your plate. how plants make food animals eat. Photosynthesis, it’s so II: Chloroplasts, Thylakoids, Light, and Chlorophyll) In a lesf there’s mesophyllic And cells with chloroplasts key the issue. Chloroplasts are almost like cells every way, With their own ribosomes and own DNA. They even themselves, splitting into half. A for this organelle’s independent past. This theory has a endosymbiosis, in the 60s by Lynn Margulis. Inside the chloroplast is the stroma. It’s true in It’s in Roma. Stroma syrrounds little Each one has a hollow inside. The thylakoid’s membrane loaded with protein. ‘Cause it’s the light reactions’ It’s about using light for powering electrons, All grabbing the power in photons. The needed are are those from light, Which has the energy to make the bright. highest frequency that we can see, Is violet with wavelength 380. 380 sounds small, But next to gamma it’s rather tall. Red light shines at Isn’t the spectrum nifty? Photons are packets of energy, Both a and a particle, you see. get adsorbed by pigments. It’s it’s true, it ain’t no figment! Most important are green carotenoids, and yellow xanthophylls. Together they absorb mostly red and blue. The green is not absorbed reflects back to you. In chlorophyll the porphyrin ring. See the magnesium, which you want to sing, Magnesium, metal valence two. see those electrons rocketing through. Notice the tail, a hydrocarbon Which anchors chlorophyll into thylakoid membrane. A molecule with so pretty, In the thylakoid makes electricity! ( Part Englemann showed us the action a prism to break the sun’s into its frequencies. Shone it on filamentous algae. The algae make will stimulate bacteria. Which will grow in any oxygen – rich Bacteria loved it over and red, But hardly over green, they might as well be dead! The line of growth is a refection, of photosynthesis’ spectrum. Note won’t match, exactly, The absorption spectra of chlorophyll a b. ‘Cause the cartenoids and xanthophylls we’ve Absorb light frequencies the chlorophylls get. And in the thylakoid they cooperate, In light to synthesize carbohydrate. Consider in isolation, some light upon it (call it photoexcitation) The electrons in magnessium Jump to an excited state, it’s so once up at that level, they can only fall. Back to they started, like a bouncing ball. These falling release energy, Fluorescing as red light, as you see. But thylakoids, chlorophyll’s not alone, Instesd a photosystem chlorophyll’s home. photosystem’s parts can take photons, And use their energy to move The system’s complex does the first capture, Changing photon into electron rapture. The energized electrons like a ball, At reaction center, they jump but do not fall. A primary electron acceotor grabs them on top, And yanks them with a force center cannot stop. To oxidized reaction center we’ll return, But the details of electron flow it now time to learn. ( Part IV) ATP Synthesis in Non Cyclic Electron Flow. Non - flow is the main pathway. Much confustion relates to way Hootoystm II precedes photosystem It’s a fct to memorize, just get done! PS II’s captures a photon, Generates flow of electrons. around the world, even n Haiti, They flow to reaction center There electrons do hover, Electron passes them over. the electron transport chain, the thylakoid membrane. This chain’s a bucket brigade. Each has a similar trade. They take energized And use energy for pumping protons. From stroma to the thylakoid Packing protons into place. This makes a gradient across the membrane The protons want “out” or they’ll go But the can’t permeate. membrane won’t allow escape. There’s only port through which they travel. It’s the Synthase channel. The “-ase” you this is an enzyme. Which makes ATP all the It has binding sites for ADP and And channels diffusing protons whose knetic energy. Changes the binding conformation. Fusing with Pi which for your information. Is how thylakoids make Life’s key of energy. From respiration might feel familiar. ATP is this way in mitochondria. Chemiosomosis the name ATP production the game. ( Part V: NADPH Synthesis in Non – Cyclic Elecron Back to our electons moved the sun. Flowing Photosystem II to System I. get to PS I lke a used up battery. The chain used up their energy. But light hits PS I’s antenna Electrons boune to center. This one’s That wavelength electrons go! PS T’s electron acceptor grabs away, Leaving P700 in an way. And P700 with oxidized blues accepts the alectrons from PS II. Bak to electron from I. It now gose for a having its own fun. But powering proton pumps ain’t its It travels NADP+ reductase. An enzyme plant cells upon. pass some H and electrons. To NADP+ which reduced. It’s how NADPH produced. (Part VI: Oxygen in Non – Cycli Electron Flow) The by – product of light reactions oxygen here’s how it happens. Back PS II, it came to That P680 oxidized To replace lost P680 liberates. Electrons from which dissociates. Into one and protons two, The single O meet anoher forming O2. These protons are formed in the thylakoid Increasing proton concentration that place. So we see a side of water’s oxidation. Is of ATP creation.