Photosynthesis : part - The Light Reactions Lecture by Glenn Wolkenfeld music Lecture by Glenn Wolkenfeld Boys girls tell me what you think of this, Time for a ‘bout photosynthesis Cells use to combine water carbon dioxide. Gonna see electrons for a wild ride. Making sugar, making O¬2. But why should photosynthesis to you? Food your table. O2 rich Without photosynthesis you simple be here. ( Part I, The Picture) Photosynthesis is a redox Powered by light enzymatic action. Water’s oxidized, re duced The sugar that results drink in orange juice. The overall reaction has two First is light reactions which has as its basis. Light powered production ATP. is the by – product and you will see. Production of electron NADPH Which reducing power needed in the second phase ‘Cause NADPH, phase 2 Provides needed for reducing CO2 NADPH ia made a reduction of NADP+, whose function Is to absorb electrons and pick up Gaining energy and NADPH. 2 is the Calvin Cycle makes carbohydrate, sugar and starches and fibers on your lunch plate. It’s how plants food animals eat. Photosynthesis, so sweet! (Part II: Thylakoids, Light, and Chlorophyll) a lesf there’s mesophyllic tissue And cells with chloroplasts key to issue. Chloroplasts are almost like cells in way, their own ribosomes and their own DNA. They even reproduce themselves, splitting half. A clue for organelle’s independent past. This has a name endosymbiosis, Developed in the 60s by Margulis. Inside the chloroplast is the stroma. It’s in Tokyo. It’s true Roma. Stroma little thylakoids. Each one has a hollow space The thylakoid’s is loaded with protein. ‘Cause it’s the reactions’ scene. It’s all about using light for powering All about grabbing the in photons. The photons needed are are those light, Which has the to make the world bright. The frequency that we can see, Is violet light with wavelength nanometers, sounds small, But to gamma rays it’s rather tall. Red light shines 750. Isn’t the spectrum nifty? Photons are of light energy, Both a and a particle, you see. Phootins adsorbed by pigments. It’s real, it’s true, ain’t no figment! Most important are chlorophylls. carotenoids, and yellow xanthophylls. they absorb light, mostly red and blue. The green is absorbed it reflects back to you. In chlorophyll see the ring. See the magnesium, makes you want to sing, Magnesium, metal valence two. You’ll those electrons rocketing through. Notice the tail, a chain. anchors chlorophyll into the thylakoid membrane. A molecule with so pretty, In the thylakoid it makes Part III) Englemann showed the action spectrum. Used a prism to break the into its frequencies. Shone it on spirogyra, algae. The O2 make will stimulate bacteria. Which will grow in any oxygen – rich Bacteria loved over blue and red, But hardly grew over green, they might as well dead! The of growth is a refection, of photosynthesis’ action spectrum. Note won’t match, exactly, The absorption spectra of chlorophyll a b. ‘Cause the cartenoids xanthophylls we’ve met, Absorb frequencies the chlorophylls can’t get. And the thylakoid they all cooperate, using light to synthesize carbohydrate. Consider chlorophyll isolation, Shine some light upon it it photoexcitation) valence electrons in magnessium Jump to an excited state, it’s fun! But up at that level, they can only fall. Back where they started, like a bouncing ball. These falling alectrons, energy, as red light, as you can see. But in thylakoids, chlorophyll’s not Instesd a photosystem is chlorophyll’s photosystem’s parts can take photons, And use energy to move electrons. The system’s antenna complex does the first photon anergy into electron rapture. energized electrons bounce like a ball, At the center, they jump but do not fall. A primary electron acceotor grabs them on top, yanks them with a force reaction center cannot stop. To this reaction center we’ll return, But the details of electron flow it now is time learn. Part IV) ATP Synthesis in Non – Cyclic Electron Flow. Non - cycle flow is the pathway. Much confustion relates to the II precedes photosystem I. a fct to memorize, just get it done! PS II’s antenna captures photon, a flow of electrons. All around world, even n Haiti, They flow reaction center P680. electrons do not hover, Electron acceptor passes over. To electron transport chain, In the membrane. This chain’s like bucket brigade. Each has a similar trade. They take energized And use their energy for pumping From stroma the thylakoid space. protons into that place. This makes a gradient across the thylkoid The want “out” or they’ll go insane. But the protons permeate. The membrane allow escape. There’s only one port through they travel. the ATP Synthase channel. The “-ase” tells you this an enzyme. Which makes ATP all time. It binding sites for ADP and Pi, And channels for diffusing protons whose knetic Changes the sites’ conformation. ADP with Pi which for your information. Is how thylakoids make Life’s key form energy. From respiration this might feel ATP is made way in mitochondria. Chemiosomosis is name ATP is the game. ( Part V: NADPH in Non – Cyclic Elecron Flow) Back to electons moved by the sun. Flowing from Photosystem to System I. They get to PS I lke used up battery. The transport chain used their energy. But when light hits PS I’s Electrons boune to center. one’s P700, That makes electrons go! PS T’s electron grabs them away, Leaving P700 an oxidized way. And P700 its oxidized blues accepts the alectrons from PS II. Bak electron from PS I. It now gose for a ride, its own fun. powering proton pumps ain’t its fate. It travels NADP+ reductase. An plant cells count upon. To pass some H and To NADP+ which gets It’s how get produced. (Part VI: Production in Non – Cycli Electron Flow) The by – of these light reactions Is oxygen how it happens. Back PS II, it came to That got oxidized To replace lost electrons P680 Electrons from water which Into one oxygen and two, The O will meet anoher forming O2. These protons are in the thylakoid space. Increasing proton concentration in place. we see a side effect of water’s oxidation. Is enhancement ATP creation.