Photosynthesis : part A The Light Reactions Amusical Lecture by Glenn A music by Glenn Wolkenfeld Boys and girls tell me what you of this, Time a lesson ‘bout photosynthesis Cells use light combine water carbon dioxide. Gonna electrons going for a wild ride. Making sugar, making O¬2. why should photosynthesis matter to you? on your table. O2 atmosphere. Without photosynthesis simple wouldn’d be here. ( Part I, Big Picture) Photosynthesis a redox reaction. Powered by light using enzymatic Water’s oxidized, re duced The sugar that you drink in orange juice. The overall reaction has phases. First the light reactions which has as its basis. Light powered production of O2 is by – product and you will see. of electron carrier NADPH Which reducing power needed in the second phase ‘Cause NADPH, phase 2 what’s needed for reducing CO2 NADPH ia by a reduction of NADP+, whose function Is to absorb electrons and up H, Gaining energy and making Phase 2 is the Calvin Cycle makes Like sugar and starches and fibers on lunch plate. It’s how plants food animals eat. Photosynthesis, so sweet! (Part II: Chloroplasts, Thylakoids, Light, and In a there’s mesophyllic tissue And with chloroplasts key to the issue. Chloroplasts are almost like cells in every With their own ribosomes and own DNA. They even reproduce themselves, splitting half. A clue for this independent past. This theory a name endosymbiosis, Developed in the 60s by Margulis. Inside the chloroplast the fluid stroma. It’s true in It’s true Roma. Stroma little thylakoids. Each one has a hollow space thylakoid’s membrane is loaded with protein. ‘Cause it’s the light reactions’ It’s all about using light for powering about grabbing the power in photons. The photons needed are are from light, Which the energy to make the world bright. The highest frequency that we can Is violet with wavelength 380. 380 nanometers, small, But next to gamma rays rather tall. light shines at 750. Isn’t the visible spectrum Photons are packets of light Both a wave and a particle, see. Phootins adsorbed by pigments. It’s real, it’s true, it ain’t no Most important are green carotenoids, and yellow xanthophylls. Together they absorb light, mostly red and green is not absorbed it reflects back to you. In chlorophyll see the porphyrin the magnesium, which makes you want to sing, Magnesium, metal with valence You’ll see those electrons rocketing Notice the tail, a hydrocarbon Which anchors chlorophyll into the thylakoid A molecule style, so pretty, In the thylakoid it makes ( III) Englemann us the action spectrum. Used a prism break the sun’s into its frequencies. Shone on spirogyra, filamentous algae. The O2 algae make will stimulate Which will grow in oxygen – rich area. loved it over blue and red, But hardly grew over green, they might as well dead! The line of growth is a of photosynthesis’ action spectrum. Note this match, exactly, absorption spectra of chlorophyll a or b. ‘Cause the and xanthophylls we’ve met, light frequencies the chlorophylls can’t get. And in thylakoid they all cooperate, using light to synthesize carbohydrate. Consider chlorophyll in some light upon it (call it photoexcitation) valence electrons in magnessium to an excited state, it’s so fun! But up at that level, they can only fall. Back to where they started, like a bouncing These falling alectrons, energy, as red light, as you can see. But in chlorophyll’s not alone, Instesd a photosystem is home. The parts can take photons, And use energy to move electrons. system’s antenna complex does the first capture, Changing photon anergy into electron The electrons bounce like a ball, At the reaction center, they jump but not fall. A primary electron grabs them from on top, And yanks with a force reaction center cannot stop. To oxidized reaction center we’ll return, But the details of electron it now is time to learn. ( Part IV) ATP Synthesis Non – Cyclic Electron Flow. Non - cycle flow the main pathway. confustion relates to the way II precedes photosystem I. It’s a fct to memorize, just get it PS antenna captures a photon, Generates a flow of All around the world, n Haiti, They flow to reaction center There electrons do not Electron passes them over. To the electron chain, In the membrane. This chain’s a bucket brigade. Each molecule has a trade. take energized electrons, And their energy for pumping protons. From to the thylakoid space. Packing protons that place. makes a gradient across the thylkoid membrane The protons want “out” or they’ll insane. But the protons permeate. The membrane won’t allow There’s one port through which they travel. It’s ATP Synthase channel. The tells you this is an enzyme. Which makes ATP the time. It has binding sites for and Pi, And channels diffusing protons whose knetic energy. Changes the binding sites’ Fusing with Pi which for your information. Is how thylakoids make Life’s key form energy. From this might feel familiar. is made this way in mitochondria. Chemiosomosis is name ATP production is the ( Part NADPH Synthesis in Non – Cyclic Elecron Flow) Back to our electons moved the sun. Flowing from Photosystem II to System They get to PS lke a used up battery. The transport chain up their energy. But when light PS I’s antenna boune to reaction center. This P700, That wavelength electrons go! PS T’s acceptor grabs them away, Leaving in an oxidized way. And P700 with its oxidized blues accepts the alectrons from PS Bak electron from PS I. It gose for a ride, having its own fun. powering proton pumps ain’t its fate. travels to NADP+ reductase. An enzyme plant cells count pass some H and electrons. To NADP+ which gets It’s how NADPH get (Part Oxygen Production in Non – Cycli Electron Flow) The by – product these light reactions Is oxygen how it happens. PS II, it came to arise, That P680 oxidized To replace lost electrons P680 Electrons water which dissociates. Into one oxygen and two, The single O will meet anoher forming These protons formed in the thylakoid space. Increasing proton concentration that place. So see a side effect of water’s oxidation. Is enhancement ATP creation.